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Wednesday, May 20, 2020 | History

2 edition of Demixing of aqueous polymer two-phase systems in low gravity found in the catalog.

Demixing of aqueous polymer two-phase systems in low gravity

Demixing of aqueous polymer two-phase systems in low gravity

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  • 22 Currently reading

Published by National Aeronautics and Space Administration in [Washington, DC .
Written in English

    Subjects:
  • Aqueous polymeric coatings.,
  • Polymerization.,
  • Reduced gravity environments.

  • Edition Notes

    StatementB. Bamberger ... [et al.].
    SeriesNASA-TM -- 101141., NASA technical memorandum -- 101141.
    ContributionsBamberger, S., United States. National Aeronautics and Space Administration.
    The Physical Object
    FormatMicroform
    Pagination1 v.
    ID Numbers
    Open LibraryOL15280114M

    Oleaginous algae are nowadays of significance for industrial biotechnology applications and for the welfare of society. Tremendous efforts have been put into the development of economically feasible and effective downstream processing techniques in algae research. Currently, Liquid Biphasic Systems (LBSs) are receiving much attention from academia and industry for their Author: Hui Yi Leong, Chih Kai Chang, Jun Wei Lim, Pau Loke Show, Dong Qiang Lin, Jo Shu Chang. In two-phase fluid flow in is convenient to use the mixture density. The mixture density of the two-phase flow which is used to calculate pressure drop. The mixture density in a two-phase fluid flow is defined as: Reactor Physics and Thermal Hydraulics: J. R. Lamarsh, Introduction to Nuclear Reactor Theory, 2nd ed., Addison-Wesley, Reading, MA.

    High Throughput Studies of Polymer and Solution Structure on the Phase Separation of Thermoresponsive Polymers Hanbin Mao, Chunmei Li, Yanjie Zhang, Steven Furyk, Paul S. Cremer and David E. Bergbreiter Macromolecules 37 () pdf Probing the Mechanism of Aqueous Two-Phase System Formation for alpha-Elastin On-Chip. In an aqueous salt solution, normal boiling (i.e. production of low salinity vapor) occurs when the fluid crosses the two-phase curve below critical pressure and temperature. However, if the fluid crosses the two-phase curve at a pressure and temperature above the critical point, a high-salinity brine condenses out of the fluid.

    applications. With regard to these problems, aqueous two-phase systems (ATPS) appear to be a powerful and attractive candidate for separating and purifying a variety of biological products []. ATPS partitioning is generally obtained by the incompatibility between aqueous solutions of two polymers (PEG, dextran, etc) or a polymer and a salt. The point C shows the net membrane at which the two-phase region is in equilibrium, where point S is the solid (polymer rich) phase and point L is the liquid (polymer-poor) phase. On the line S-L, the position C is used to determine the membrane porosity [7, 8].


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Demixing of aqueous polymer two-phase systems in low gravity Download PDF EPUB FB2

Get this from a library. Demixing of aqueous polymer two-phase systems in low gravity. [B Bamberger; United States. National Aeronautics and Space Administration.;]. The one-g demixing of systems possessing equal densities has been studied.

We have also studied the demixing of PEG/dextran systems in low gravity on KC aircraft and during one Shuttle mid-deck experiment. The demixing rate data from these experiments have been analysed by two different by: 9.

Electrokinetic demixing of aqueous two-phase polymer/salt systems Article in Electrophoresis 26(1) January with 8 Reads How we measure 'reads'. The kinetics of phase demixing in an aqueous two-phase system (ATPS) consisting of PEG/sodium citrate, with various PEG molecular weights (,and ) was investigated at 25 degrees C.

Phase Partitioning in Space and on Earth. Authors; Authors and affiliations In low-gravity, demixing has been shown to occur primarily by coalescence. Polymer surface coatings, developed to control localization of demixed phases in low-g, have been found to control electroosmosis which adversely affects electrophoretic separation processes Cited by: 7.

Purchase Aqueous Two-Phase Systems, Volume - 1st Edition. Print Book & E-Book. ISBNThe nature of phase separation in aqueous two-polymer systems Ake Gustafsson and H&kan Wennerstr6m Department of Physical Chemistry 1, Chemical Centre, P.O.B.

S, O0 Lund, Sweden and Folke Tjerneld Department of Biochemistry, Chemical Centre, P.O.B. S O0 Lund, Sweden (Received 10 February ; revised 23 April ) Aqueous systems Cited by: Such demixing typically takes place already at low polymer concentrations, often below 10% by mass.

In water, such systems are often named aqueous two-phase systems (ATPS), with a so-called water/water interface separating the two phases.

ATPS have a long history. Beijerinck observed already in the phase separation in aqueous mixtures of. total volume of mL. All two-phase systems had the polymer compositions indicated in Table 1 and salt composition of M NaCl in M sodium phosphate buffer (NaPB), pH Partitioning experiments.

An automated instrument for performing aqueous two-phase partitioning, Automated Signature. Various attributes of the three major types of aqueous polymer two-phase systems are noted in Table In general, polymer-polymer systems are more expensive, can have viscous phases, and offer reasonable protein target solubility (i.e., capacity) of 5–10 g protein/L in the target-containing by: 1.

Aqueous two-phase systems. Methods and protocols Hatti-Kaul R. Categories: Biology\\Biochemistry. Year: polymer aqueous two phases dextran proteins affinity cell separation phase system You can write a book review and share your experiences.

Other readers will always be interested in. Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia.

Although, the partition behavior involved in the method is complex Cited by: Abstract. Aqueous polymer-polymer two-phase systems provide a powerful method for separating biomolecules by extraction. When a complex mixture of biomolecules (e.g., a fermentation broth or a solution of lysed cells) is added to such a system, biomolecules partition uniquely between the two phases, achieving separation.

Liquid–liquid extraction (LLE), also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar).

There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to. Cholinium-based ionic liquids made of aminoate anions ([Ch][AA] ILs) have been proven to have low toxicity and to be readily biodegradable.

This class of ILs has great potential as a novel phase-forming component for aqueous two-phase system (ATPS) used in protein separation.

In this study, environmentally benign ATPSs were prepared by using a series of [Ch][AA] ILs Cited by: Ternary systems composed of water-miscible ILs, inorganic/ organic salts and water, known as IL-based aqueous biphasic systems (ABSs), have been proposed in the last decade, mainly for use in liquid–liquid extraction,11 Conventional ABSs are formed when two different polymers, or a polymer and a salt.

Aqueous Two Phase Systems for the Recovery of Biomolecules – A Review relative size of the drops[7]. Phosphates and sulfates are the commonly used salts in polymer/salt ATPS. But this leads to high phosphate and sulfate TLLconcentration in the effluent streams and thehence an environmental concern.

Nowadays. Upon demixing, an aqueous solution of a polyelectrolyte and an incompatible neutral polymer yields two phases separated by an interface with an ultralow tension. Here, both in theory and experiment, we study this interfacial tension in detail: how it scales with the concentrations of the polymers in the two phases and how it is affected by the interfacial difference in the electrical Cited by: 6.

Preparation of aqueous two-phase system Aqueous two-phase systems were prepared from stock solutions of poly (ethylene glycol) of about 40% (w/w) and maltodextrin of about 35% (w/w). Partitioning of soluble proteins from shrimp waste was carried out in PEG/ maltodextrin system.

Phase systems were prepared in. Available in the National Library of Australia collection. Format: Book; xii, p.: ill. (some col.) ; 24 cm. polymer [12–14]and the polymer can be recycled [15]. The cloud point temperature of these aqueous polymer solutions is a linear function of the mass fraction of PO in the copoly-mer [16,17].

In this work, the extraction of endo-PG and total pro-tein from a K. marxianus fermentation broth in an aque-ous two-phase system containing.Aqueous two-phase systems of the polymer-salt type have several advantages over the polymer-polymer type: the larger relative size of the drops, larger differences in density, greater selectivity, lower viscosity and lower cost; besides, it is more difficult to use systems with two polymers on an industrial scale due to high viscosity and high.Aqueous two-phase systems (ATPS) have been reported as an attractive biocompatible extraction system for recovery and purification of biological products.

In this work, the implementation, characterization, and optimization (operational and economic) of invertase extraction from spent brewery yeast in a semi-automatized pilot plant using ATPS Cited by: 1.